Basics of gc-ms pdf




















Bilal Ahmad Dar. A short summary of this paper. Download Download PDF. Translate PDF. Rather et al. Rather1, Shahnawaz. Sofi 1 , Bilal. Dar 1 , Bashir. Received on: ; Revised on: ; Accepted on ABSTRACT In the current study, the mono and sesquiterpenoid composition in the leaves and stem of Artemisia amygdalina Decne is tracked by probing the contents of individual mono and sesequiterpenoids in the leaf and stem essential oils obtained by hydrodistillation using a combination of capillary GC-FID and GC-MS analytical techniques.

The analysis led to the identification of 25 components in the leaf and 32 components in the stem essential oils. The leaf essential oil is dominated by monoterpene hydrocarbons The principal components were sabinene The stem essential oil is dominated by monoterpene hydrocarbons This genus belongs 2. Extraction of the Essential Oils to a useful group of medicinal and aromatic plants comprising of about species The shade dried leaves and stem of the plant were finely chopped and most of which are distributed in North America, Europe, Asia and North Africa [1,2].

The oil obtained was dried over anhydrous sodium sulphate and Western Himalayan region [2]. Artemisia species are popular from the chemical, stored at 4 0C in a sealed vial until analysis. The genus Artemisia is known to contain many Gas chromatography was carried out on Perkin Elmer auto system XL Gas bioactive compounds such as artemisinin which possesses antimalarial and cytotoxic Chromatograph series equipped with flame ionization detector FID activity against tumor cells [ 4 ], arglabin another bioactive molecule is used and head space analyzer using a fused silica capillary RTX -5 Column for treating certain types of cancer in the former USSR [5].

It is a tall, stout stemmed leafy aromatic perennial herb with simple serrate leaves which are hoary tomentose beneath and glabrous green above, 2. In the traditional system of GC- MS analysis was carried on a Varian Gas Chromatograph series medicine, the plant is used by locals for the treatment of cough, cold, worms fitted with a VF 5 ms fused silica capillary column 60m x 0.

It is also used as vermifuge [6]. Despite being a very important film thickness 0. The plant as carrier gas at 1. The relative percentages of the individual components were calculated based on GC peak area FID response without using correction factors.

Plant Material The plant material of Artemisia amygdalina was collected from the Gurez 3. A voucher specimen voucher number, amygdalina are shown in table-1, in order of their elution from RTX-5 KASH of the plant material was deposited in the Kashmir column.

The oil yields calculated on dry weight basis were 0. The total ion chromatograms TIC of the two essential oils are shown in fig-1 and fig The leaf essential oil is dominated by the presence of monoterpene hydro- Bashir A Ganai carbons Sesquiterpene University of Kashmir, hydrocarbons constituted The principle components of the leaf were sabinene Journal of Pharmacy Research Vol.

Issue 6. Camphor 1. Ledene oxide —- 1. Oxygenated monoterpenes constituted The graphical 69 39 91 representation of various mono and sesquiterpenes in the two essential oils is 77 shown in fig The chemical structures of the Major compounds present in 29 43 15 74 86 the two essential oils along with their corresponding mass spectra are shown in 0 figures 4 to Compound class distribution in the leaf and stem of A. Figure Structure and mass spectrum of 1,8-cineole. In conclusion, the present report of the chemical profile of the essential oil of Artemisia amygdalina which is the first ever report provides a further in- 95 depth information about the chemo diversity in the chemical composition of the essential oil of the genus Artemisia.

Mass of Materials. Mass Number and Isotope. Isotopic Abundance of Carbon Atoms. Isotope Abundance Table. Molecular Weight. Introduction: Types of Ions. Example of Mass Spectrum. Base Peak. Molecular Ion Peak. Fragment Ion. Isotope Peak. Ionization Modes: EI. Three Ionization Modes. Features of EI. Molecular Ion Produced by EI. Fragment Ion Produced by EI. Full View of EI Process.

Producing EI Mass Spectrum. Electron Energy and Mass Spectrum. Ionization Modes: PCI. Features of PCI. Spectrum of PCI. Appropriate Conditions of PCI. Ionization Process of PCI. Reactions Ionizing Sample Molecules. Proton Affinity. Comparison of Reagent Gasses.

Ionization Modes:NCI. Features of NCI. Spectrum of NCI. Ionization Process of NCI. Molecular Peak and Fragmentation. Negative ion Production Processes.

Useful Application Fields. Hardware: Ion Source. Overview of Ion Source. Structure of Ion Source. To Control Ionization. The Role of Magnets. Comparison of Sources.

Hardware: Mass Analyzer. Overview of Mass Analyzer.



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