Real, A. Winter, A. Walton, and S. Vaccination with the plasminogen activator from Streptococcus uberis induces an inhibitory response and protects against experimental infection in the dairy cow. Vaccine 17 : Streptococcus uberis acquires plasmin activity following growth in the presence of bovine plasminogen through the action of its specific plasminogen activator. Hodgkinson, and R. The interaction of Streptococcus dysgalactiae with plasmin and plasminogen.
Lincoln, R. Characterisation of the interaction of bovine plasmin with Streptococcus uberis. Maguin, E. Ehrlich, and A. Efficient insertional mutagenesis in lactococci and other gram-positive bacteria. McCoy, H. Broder, and R. Streptokinases produced by pathogenic group C streptococci demonstrate species-specific plasminogen activation. Rosey, E. Lincoln, P. Ward, R. Yancey, Jr. PauA: a novel plasminogen activator from Streptococcus uberis. Smith, A. Kitt, P. Ward, and J.
Isolation and characterization of a mutant strain of Streptococcus uberis, which fails to utilize a plasmin derived beta-casein peptide for the acquisition of methionine. Ward, T. Field, C. Jones, R. Lincoln, and J. MtuA, a lipoprotein receptor antigen from Streptococcus uberis , is responsible for acquisition of manganese during growth in milk and is essential for infection of the lactating bovine mammary gland.
Thomas, L. Haider, A. Hill, and R. Pathologic findings of experimentally induced Streptococcus uberis infection in the mammary gland of cows. Ward, P. Characterization of PauB, a novel broad-spectrum plasminogen activator from Streptococcus uberis. Full text links Read article at publisher's site DOI : Smart citations by scite. The number of the statements may be higher than the number of citations provided by EuropePMC if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
Explore citation contexts and check if this article has been supported or disputed. Sequence characterisation and novel insights into bovine mastitis-associated Streptococcus uberis in dairy herds. Molecular epidemiology of mastitis pathogens of dairy cattle and comparative relevance to humans. Data Data behind the article This data has been text mined from the article, or deposited into data resources.
BioStudies: supplemental material and supporting data. Similar Articles To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation. MtuA, a lipoprotein receptor antigen from Streptococcus uberis, is responsible for acquisition of manganese during growth in milk and is essential for infection of the lactating bovine mammary gland. Bovine intramammary infection associated immunogenic surface proteins of Streptococcus uberis.
Potential virulence factors of Streptococcus dysgalactiae associated with bovine mastitis. Streptococcus uberis: a permanent barrier to the control of bovine mastitis?
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Subsequent analysis revealed that strain C differed from the others in that it did not produce detectable quantities of the plasminogen activator. Following incubation of culture filtrate from this strain with bovine plasminogen no plasmin activity was detected Fig.
As previously reported [ 2 , 3 ], none of the strains was able to activate human plasminogen and this was also reflected by the corresponding low level of plasmin activity associated with S. Activation of either bovine or human plasminogen by culture supernatants from four strains of S.
Culture supernatants from S. The presence of plasmin is indicated by a caseinolytic zone in the skimmed milk containing agarose. The three strains which showed high levels of bound plasmin activity following growth in BHI containing bovine plasminogen also exhibited similar or greater levels of bound plasmin activity following direct incubation with bovine plasmin Fig. Strain C which did not show high levels of bound plasmin activity following growth in BHI containing bovine plasminogen did, however, bind the enzyme at a level similar to that seen on the other strains following incubation with plasmin directly Fig.
This observation along with the previous data suggests that the failure of this strain to bind bovine plasmin following growth in BHI containing bovine plasminogen resulted from the inability to activate the zymogen rather than an inability to bind the activated protein.
This finding is in agreement with that of Malke et al. Cell-associated plasmin activity following incubation of S. Data repreents the mean optical density nm values obtained from at least three determinations following incubation with either human open bars or bovine solid bars plasmin. In order to substantiate this interpretation, strain C was grown in BHI containing bovine plasminogen and either urokinase or culture filtrate from S.
Following growth in media containing a source of plasminogen activator, strain C was able to bind bovine plasmin Fig. The infectivity of strain C for the bovine mammary gland is not known, however, such a strain may be useful in the determination of the role of plasminogen activation in the pathogenesis of bovine mastitis caused by S.
In contrast to the ability to activate bovine plasminogen, none of the strains activated human plasmin Fig. Somewhat surprisingly, following incubation of washed cells in human plasmin directly, each strain exhibited around five-fold less bound protease activity following incubation with human plasmin compared to bovine plasmin Fig.
This latter observation is in contrast to those of others in which the levels at which streptococci bound plasmin from target and non-target species was similar [ 4 ] and may indicate a level of specificity in the plasmin-bacterium interaction for S. These observations are likely to account for the inability of S. In order to determine whether S. Following incubation with bovine plasminogen, and in the absence of subsequent activation of any bound plasminogen with urokinase, no protease activity was detected in association with the bacterial cells data not shown.
Following incubation of similarly treated cells with urokinase, only trace levels of plasmin were detected in association with any of the strains. The absorbance values obtained never exceeded 0. This observation is similar to that of McCoy et al. It would appear, therefore, that S.
This is the first report to indicate that S. The acquisition of this activity may have pleiotrophic effects on the pathogenesis of infection, any or all of which may be relevant to infection and induction of mastitis in the ruminant mammary gland. Hillerton E. Dairy Res.
Google Scholar. Leigh J. FEMS Microbiol. McCoy H. Ohkuni H. Walter, F. Nucleic Acids Res. Lottenberg, and K. Cloning, expression, sequence analysis, and characterization of streptokinases secreted by porcine and equine isolates of Streptococcus equisimilis. Calvinho, L. Almeida, and S. Potential virulence factors of Streptococcus dysgalactiae associated with bovine mastitis.
Coligan, J. Dunn, H. Ploegh, D. Speicher, and P. Wingfield ed. Current protocols in protein science. Collen, D. Staphylokinase: a potent, uniquely fibrin-selective thrombolytic agent. Edberg, S. Gam, C. Bottenbley, and J. Rapid spot test for the determination of esculin hydrolysis.
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Hill, A. DNA fingerprinting of Streptococcus uberis: a useful tool for epidemiology of bovine mastitis. Johnsen, L. Purification and cloning of a streptokinase from Streptococcus uberis. Johnston, K. Solid and fluid phase assays for bacterial plasminogen activators. Methods 18 : — Kitt, A. The auxotrophic nature of Streptococcus uberis. The acquisition of essential acids from plasmin derived casein peptides.
Laemmli, U. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature : — Leigh, J.
Purification of a plasminogen activator from Streptococcus uberis. FEMS Microbiol. Streptococcus uberis: a permanent barrier to the control of bovine mastitis? Hodgkinson, and R. The interaction of Streptococcus dysgalactiae with plasmin and plasminogen. Streptococcus uberis acquires plasmin activity following growth in the presence of bovine plasminogen through the action of its specific plasminogen activator. Lottenberg, R. DesJardin, H.
Wang, and M. Streptokinase-producing streptococci grown in human plasma acquire unregulated cell-associated plasmin activity. Minning-Wenz, and M. Capturing host plasmin ogen : a common mechanism for invasive pathogens? Trends Microbiol. McClintock, D. The mechanism of activation of human plasminogen by streptokinase. McCoy, H. Broder, and R. Streptokinases produced by pathogenic group C streptococci demonstrate species-specific plasminogen activation. Nielsen, H.
Engelbrecht, S. Brunak, and G. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. Nowicki, S. Minning-Wenz, K. Johnston, and R. Characterization of a novel streptokinase produced by Streptococcus equisimilis of non-human origin. Oliver, S. Almeida, and L. Virulence factors of Streptococcus uberis isolated from cows with mastitis.
Reihe B 45 : — Reddy, K. Mechanism of activation of human plasminogen by streptokinase. Similar Articles To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation. Streptococcus uberis acquires plasmin activity following growth in the presence of bovine plasminogen through the action of its specific plasminogen activator.
Purification of a plasminogen activator from Streptococcus uberis. The interaction of Streptococcus dysgalactiae with plasmin and plasminogen. Molecular mechanisms of plasminogen activation: bacterial cofactors provide clues.
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